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Prof Allan E Herbison FRSNZ

Prof Allan E Herbison, FRSNZ

Professor of Neuroendocrinology

Wellcome Trust Senior Research Fellow


Office Phone: +44 (0) 1223 333 839

Research Interests

We are focused upon understanding the properties and functioning of the key neural populations controlling fertility in mammals; the gonadotropin-releasing hormone (GnRH) neurons and the kisspeptin neurons. Together, these cells generate the “pulse” and “surge” patterns of hormone secretion that are responsible for the initiation of puberty and the subsequent maintenance of reproductive function in adult males and females.

Our key questions are -

  • How does this neural circuitry produce the abrupt episodes of GnRH secretion that generate pules of reproductive hormone secretion?
  • How can this same circuitry generate a completely different “surge” pattern of GnRH secretion at the mid-point of the female cycle to trigger ovulation?
  • How do steroids such as estrogen and progesterone modulate this neural circuitry?
  • What goes wrong in this neural circuitry in conditions of infertility such as polycystic ovary syndrome (PCOS)?

Studies are undertaken in a variety of genetically-modified mouse models using tissue clearing and expansion microscopy, single cell electrophysiology and calcium imaging in acute brain slices in vitro, RNAseq gene profiling, in vivo imaging of the activity of selected neurons and neural populations in freely-behaving mice, and in vivo CRISPR-based gene editing.

 

Funding

Wellcome Trust

 

Group Members

Su Young Han (Postdoctoral Fellow)
Maria Parado Navarro (Senior Research Technician)
Shel Hwa Yeo (Postdoctoral Fellow)

Key Publications

Herbison AE (2018) The gonadotropin-releasing hormone pulse generator. Endocrinology, 159, 3723-3736. doi:10:1210/en.2018-00653

Clarkson, J., Han, S. Y., Piet, R., McLennan, T., Kane, G. M., Ng, J., Porteous, R., … Herbison, A. E. (2017). Definition of the hypothalamic GnRH pulse generator in mice. Proceedings of the National Academy of Sciences, 114(47), E10216-E10223. doi: 10.1073/pnas.1713897114

Herbison, A. E. (2016). Control of puberty onset and fertility by gonadotropin-releasing hormone neurons. Nature Reviews Endocrinology, 12, 452-466. doi: 10.1038/nrendo.2016.70

Han, S. Y., McLennan, T., Czieselsky, K., & Herbison, A. E. (2015). Selective optogenetic activation of arcuate kisspeptin neurons generates pulsatile luteinizing hormone secretion. Proceedings of the National Academy of Sciences, 112(42), 13109-13114. doi: 10.1073/pnas.1512243112

Campos, P., & Herbison, A. E. (2014). Optogenetic activation of GnRH neurons reveals minimal requirements for pulsatile luteinizing hormone secretion. Proceedings of the National Academy of Sciences, 111(51), 18387-18392. doi: 10.1073/pnas.1415226112

Kirilov, M., Clarkson, J., Liu, X., Roa, J., Campos, P., Porteous, R., … Herbison, A. E. (2013). Dependence of fertility on kisspeptin—Gpr54 signalling at the GnRH neuron. Nature Communications, 4, 2492. doi: 10.1038/ncomms3492

Plain English

One in five couple suffer from being unable to get pregnant. In about one third of situations, this occurs because the brain is not controlling the activity of the ovaries correctly. Our research is investigating exactly how cells in the brain work together to send the correct signals to the ovary. Understanding this process is critical to being able to generate new treatments aimed at helping infertile couples.

Multiple GnRH neurons surrounding the OVLT in a mouse

GCaMP6 fiber photometry recording of arcuate kisspeptin neurons and pulsatile LH levels in an adult female mouse

GCaMP6 calcium concentration recordings from multiple arcuate kisspeptin neurons