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Dr Clare Buckley

My lab uses optogenetics and live confocal imaging of the zebrafish neural tube to study how the polarity of individual cells drives the organisation of a whole vertebrate organ.
Dr Clare  Buckley

Royal Society Dorothy Hodgkin Research Fellow

Clare Buckley is accepting applications for PhD students.


Office Phone: +44 (0) 1223 3 33766

Biography:

Clare received her undergraduate degree in Biological Sciences from the University of Oxford. Her PhD in Neuroscience was from the laboratory of Robin Franklin at the University of Cambridge in collaboration with a small biotechnology company, Summit Plc. (previously DanioLabs). Clare carried out her postdoctoral work in the laboratory of Jon Clarke at King’s College London, during which time she held a short-term EMBO fellowship at Orion Weiner’s laboratory at the University of California, San Francisco to develop the Phytochrome system for use in zebrafish. She has recently been awarded a Royal Society Dorothy Hodgkin Research Fellowship to start her own laboratory at the Department of Physiology, Development and Neuroscience at the University of Cambridge.

Research Interests

My lab uses optogenetics and live confocal imaging of the zebrafish neural tube to study how the polarity of individual cells drives the organisation of a whole vertebrate organ. Answering this fundamental biological question is important for understanding body patterning during development; how does tissue-wide epithelial organisation arise amid such dynamic cell movement and proliferation? It is also important for understanding tissue disorganisation during disease; could defects in cell polarity play a role in the loss of tissue organisation at the onset of diseases such as cancer?

In addition to high resolution in vivo imaging, we use a new optogenetic approach to manipulate polarity, signalling and division in individual cells within the developing zebrafish neural tube in vivo using light. In combination with CRISPR-mediated functional knock down experiments, this allows us to explore how cell polarity and division are linked during development such that cells can divide without disrupting the strict organization of the tissue. We also use this method to manipulate the cancer-linked phosphatidylinositol 3–kinase (PI3K) signalling pathway, which is likely key to integrating apicobasal polarity with cell division. We use this approach to investigate whether apico-basal polarity dysregulation in disease is a cause or consequence of tissue disruption.

Collaborators

Jared Toettcher, Princeton University

 

Collaborators

Key Publications

Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner OD and Clarke JDW. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell (2016) Jan 11:36(1): 117-26

Buckley CE and Clarke JDW. Establishing the plane of symmetry for lumen formation and bilateral brain formation in the zebrafish neural rod. Semin Cell Dev Biol. (2014) Jul;31: 100-5

Buckley CE, Ren X, Ward LC, Girdler GC, Araya C, Green MJ, Clark BS, Link BA and Clarke JDW. Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod. EMBO J. (2013) Jan 9;32(1): 30-44

Buckley CE, Marguerie A, Roach AG, Goldsmith P, Fleming A, Alderton WK and Franklin RJM. Drug reprofiling using zebrafish identifies novel compounds with potential pro-myelination effects. Neuropharmacology (2010) Sep;59(3):149-59

Buckley CE, Marguerie A, Alderton WK and Franklin RJM. Temporal Dynamics of Myelination in the Zebrafish Spinal Cord. GLIA (2010) 58:802–812.

Buckley CE, Goldsmith P and Franklin RJM. Zebrafish Myelination: A Transparent Model for Remyelination? DMM (2008) 1: 221-228

 

Above: Mosaically labelled neuroepithelial cells in the developing zebrafish neural tube