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Regulation of neural stem cell behaviour by long non-coding RNAs

Supervisor: Andrea Brand

Thousands of long noncoding RNAs (lncRNAs) are expressed in spatially and temporally restricted patterns, but the role of the majority of these transcripts is still unknown. lncRNAs are thought to function, at least in part, through the modulation of chromatin state. To investigate lncRNA - chromatin interactions in vivo we developed RNA-DamID, a novel approach that can detect genome-wide binding of lncRNAs in a cell-type specific manner with high sensitivity. We have identified a small group of lncRNAs that are differentially expressed in neural stem cells. The project will investigate the function of a subset of lncRNAs that we have discovered to be differentially expressed in neural stem cells as they move from quiescence to proliferation.

 

Relevant references

Cheetham, S.W. and Brand, A.H. (in press). Cell-type-specific assembly of the dosage compensation complex identified by lncRNA-DamID. Nature Structural and Molecular Biology.

Marshall, O.J. and Brand, A.H. (in press). Novel changes in chromatin state during neural development revealed by in vivo cell-type specific profiling. Nature Communications.

Marshall O.J., Southall T.D., Cheetham S.W. and Brand A.H. (2016). Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing. Nature Protocols 11(9), 1586-1598.