Prof Roger C Thomas
Emeritus Professor of Physiology FRS (1989), Former Fellow of Downing College (1996-2006), Tel: 01223 333869, Fax: 01223 333840, E-mail: rct26@cam.ac.uk
Current Research
- Measuring the coupling between calcium extrusion and proton uptake by the plasma membrane calcium ATPase of snail neurones.
- Calcium gradients and stores inside large nerve cells.
The silanization technique I use is described here.
Past Research
Chloride channels and electrogenic sodium pump in snail nerve cells, dye marking in mammal CNS, sodium and pH-sensitive recessed-tip glass microelectrodes, intracellular pH regulation in snail neurones and other cells, proton channels.
Selected Publications
R. C. Thomas (2002) The effects of HCl and CaCl2 injections on intracellular calcium and pH in voltage-clamped snail (Helix aspersa) neurons. J. Gen. Physiol. 120: 567-579.
R. C. Thomas, S E. Pagnotta, & A. Nistri (2003) Whole-cell recording of intracellular pH with silanized and oiled patch-type single or double-barreled microelectrodes. Pflugers Archiv 447: 259-265.
R. C. Thomas (2005) Apparent calcium gradients inside snail neurones detected with a Ca2+-sensitive microelectrode. J. Physiol. Biochem. 61: 92.
R. C. Thomas & M. Postma (2007) Dynamic and static calcium gradients inside large snail (Helix aspersa) neurones detected with calcium-sensitive microelectrodes. Cell Calcium 41: 365-378.
R. C. Thomas (2009) The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded. J. Physiol. 587.2: 315-327.
R. C. Thomas (2010) Intracellular calcium-sensitive microelectrodes. In: Calcium Measurement Methods ed. A. Verkhratsky and O. H. Petersen, Humana Press, pp 119-125.
R. C. Thomas (2011) The Ca2+:H+ coupling ratio of the plasma membrane calcium ATPase in neurones is little sensitive to changes in external or internal pH. Cell Calcium 49: 357-364.
