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Jeremy Skepper PhD

Jeremy Skepper, PhD

Senior Technical Officer and Technical Director of The Multi-Imaging Centre


Office Phone: +44 (0) 1223 333774, Fax: +44 (0) 1223 333786/840

Research Interests

The MIC has acted as a focus for interdepartmental collaborations between departments of the school of biological sciences in biological electron microscopy for 44 years and for ex vivo, in vivo and in vitro confocal microscopy for 14 years.
Over the last 5 years more and more non-biological departments are requiring the use of electron microscopy to study biomaterials. Frequently they require modest resolution and high contrast for which the MIC instrument is ideal (Zhai et al, 2004, Hansen et al, 2005).
More groups are interested in the interface between biomaterials and biological structures (Porter et al, 2003 and Porter et al, 2004). These types of studies are particularly challenging, as they require the preparation of thin sections at the interface between very hard and very soft materials. The MIC has developed methods based on ultramicrotomy at ambient and at low temperature. They have successfully sectioned the interface between a biological film (bacterial and algae) and an underlying quartzite particle. The hardness of quartzite exceeds that of glass and approaches that of diamond.

My own interests are: Cardiovascular Ultrastructure, Neuroanatomy and Nanoparticle Cytotoxicity and the development of new imaging modalities.

Main sources of funding: BBSRC, Wellcome Trust, EPSRC and DTI

Colleagues

Professor Graham Burton
Dr Sue Jones
Dr Arih Lew
Dr Teresa Tiffert

Main collaborators

Professor Athene Donald (Cavendish Laboratories, Cambridge)
Dr Paul Midgley (HREM, Materials sciences, Cambridge)
Dr Alice Warley (KCL)
Dr Alex Porter (Imperial College London)
Dr Mahiri Gass (SuperSTEM)
Dr Clemens Kaminski (Chemical Engineering, Cambridge)
Professor Chris Dobson (Chemistry Cambridge)
Dr Andrew Grace (Papworth Hospital and Biochemistry, Cambridge)
Professor Chris Huang (PDN Cambridge)
Dr Jonathan Gillard (Radiology Cambridge)
Dr Peter Maycox (GlaxoSmithKlein)
Professor Cathy Shannahan (KCL)
Dr Anthony Davenport (Clinical Pharmacology, Cambridge)
Dr Mihaly Posfai (Dept of Earth and Environmental Sciences, Pannon, Hungary)

Key Publications

Muller KH, Skepper JN, Posfai M, Trevedi R, Howarth S, Corot C, Lancelot E, Thompson PW, Brown AP, Gillard JH, (2007), Effect of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) on human monocyte-macrophages in vitro, Biomaterials,  28, 1629-1642

Critchlow HM, Maycox PR, Skepper JN, Krylova O, (2006), Clozapine and haloperidol differentially regulate dendritic spine formation and synaptogenesis in rat hippocampal neurons, Molecular & Cellular Neuroscience, 32, 356-365

Porter AE,  Muller K, Skepper JN, Midgely P, Welland M, (2006), Uptake of C60 by human monocyte macrophages. Its localization and implications for toxicity: studied by high resolution electron microscopy and electron tomography, Acta Biomaterialia, 4, 409-419

Usher-Smith JA, Skepper JN, Fraser JA, Huang CLH, (2006), The effect of repetitive stimulation on cell volume and the relationship to membrane potential in amphibian skeletal muscle, Pflugers Archives, 11, 1-9

Arrebola F, Fernandez-Segura E, Campos A, Crespo PV, Skepper JN, Warley A, (2006), Changes in intracellular electrolyte concentrations during apoptosis induced by UV irradiation of human myeloblastic (U937 ) cells, American Journal of Physiology 290, C638-649

Kleinz MJ, Skepper JN, Davenport AP, (2005), Immunocytochemical localisation of the apelin receptor, APJ, to human cardiomyocytes, vascular smooth muscle and endothelial cells, Regulatory Peptides, 126, 233-240

Zhang Q, Skepper JN, Ragnauth CD, Warren DT, Roberts RG, Weissberg PL, Ellis JA, Shanahan CM, (2005), Nesprin-2 binds lamin and emerin at the nuclear envelope and is abundant in the sarcomere of skeletal muscle. Journal of Cell science 15, 673-687

Trividi RA, U-King-Im JM, Graves MJ, Cross JJ, Horsley J, Goddard MJ, Skepper JN, Quartey G, Warburton EA, Joubert I, Wang L, Kirkpatrick PJ, Brown J, Gillard, (2004), In Vivo detection of macrophages in human carotid atheroma temporal dependence of ultrasmall superparamagnetic particles of iron oxide enhanced MR imaging, Stroke, 35, 1631-1635

Hung TH, Charnock-Jones DS, Skepper JN, Burton GJ, (2004), Secretion of tumour necrosis factor-α from human placental tissues induced by hypoxia-reoxygenation caused endothelial cell activation in vitro: a potential mediator of the inflammatory response in preeclampsia, American Journal of Pathology, 164, 1049-1061

Zhai S, Hansen RK, Taylor R, Skepper JN, Sanches R, Slater NKH, (2004), Effect of freezing rates and excipients on the infectivity of a live viral vaccine during lyophilization, Biotechnology Progress, 20, 1113-1120

Porter AE, Patel N, Skepper JN, Best SM, Bonfield, (2004), Effect of sintered silicate-substituted hydroxyapatite on remodelling processes at the bone-implant interface, Biomaterials, 16, 3303-3314

Porter AE, Patel N, Skepper JN, Best SM, Bonfield W, (2003), Comparison of in vivo dissolution processes in hydroxyapatite and silicon substituted hydroxyapatite bioceramics, Biomaterials, 25, 4609-4620

Above: Ultra-small super paramagnetic iron oxide particles imaged by high resolution transmission electron microscopy. The particles have crystalline cores of magnetite 5nm in diameter surrounded by a shell of dextran. These particles are injected intravenously into patients with carotid artery disease. They accumulate at sites of inflammation and the disease state can be imaged non-invasively by MRI to determine if surgical intervention is needed.